Studies on the properties of Celluclast/Eudragit L-100 conjugate

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Dourado, F (Autor) Outra; Bastos, M (Autor) FCUP; Mota, M (Autor) Outra; Gama, FM (Autor) Outra

Revista

Título: Journal of Biotechnology

Vol. 99

Páginas: 121-131

ISSN: 0168-1656

Editora: Elsevier

Indexação

ISI Web of Knowledge - 46 Citações

Scopus - 50 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-MNK

DOI: 10.1016/s0168-1656(02)00178-5

Abstract (EN): A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid phase, thus making recycling possible. This method allows for improved stability, without major loss of specific activity. The adsorption of cellulase on Eudragit lowers the enthalpy of denaturation, but affects only slightly the denaturation temperature. The use of carbodiimide was ineffective on linking the enzymes covalently to the polymer, since the immobilization process was found to be only mediated by non-covalent forces.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11