Microbiology Laboratory

Code:

B4029

Acronym:

B4029

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2013/2014 - 1S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Cell and Molecular Biology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BCM	19	Official Study Plan until 2023/2024	1	-	6	42	162

Teaching language

Suitable for English-speaking students

Objectives

Bibliography search and organization of a database.

Discussion of scientific papers.

Improve knowledge on microbiological techniques used commonly in nowadays research.

Develop capacities to conceive structure and put in place an experimental work/design.

Organization of results obtained in the experimental work, elaboration of a poster as for presentation in a scientific meeting.

Elaboration of a manuscript.

Learning outcomes and competences

Development of capacities in cultural and molecular techniques in Microbiology

Working method

Presencial

Program

This course is mainly based on the planning and execution of a laboratorial study, treatment and analysis of the results and learning how to communicate them to the scientific community. This will be achieved by the preparation of a poster as for a scientific meeting and the elaboration of a manuscript. Culturing and genomic and/or proteomic molecular techniques will be performed in the laboratorial work. Several techniques frequently used in Microbiological studies namely Electron Microscopy, FISH, Brdu-FISH, CARD-FISH, MAR-CARD-FISH, Flow Cytometry, Fingerprinting Methods – DGGE, Pyrosequencing, q-PCR; real time-PCR and Microarrays will be studied focusing on their principles and applicability.

Mandatory literature

Reddy C. A., Beveridge T. J., Breznak J.A., Marzluf G., Schmidt T. M. & Snyder L.R.; Methods for General and Molecular Microbiology, ASM Press, 2007

Comments from the literature

Several scientific papers

Teaching methods and learning activities

Participation in laboratorial classes (DL)

Poster for a communication in a conference (P)

Preparation of a scientific paper based on the laboratorial work developed (AC)

Oral presentation of a Microbiology topic (A))

NF = 25% (RP) + 25% (P) + 25% (SP) + 25% (CP)

keywords

Health sciences
Natural sciences > Biological sciences > Biology > Microbiology

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation	Weight (%)
Participação presencial	25,00
Prova oral	25,00
Trabalho escrito	50,00
Total:	100,00

Eligibility for exams

NF = 25% (RP) + 25% (P) + 25% (SP) + 25% (CP)

Calculation formula of final grade

Participação presencial 25%

Prova oral 25%

Trabalho escrito 50%l