Neutrophil gelatinase-associated lipocalin detection using a sensitive electrochemical immunosensing approach

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Neves, MMPS (Author) Other; Nouws, HPA (Author) Other; Alice Santos Silva (Author) FFUP; Delerue Matos, C (Author) Other

Journal

Title: Sensors and Actuators, B: Chemical

Vol. 304

ISSN: 0925-4005

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00R-6S1

DOI: 10.1016/j.snb.2019.127285

Abstract (EN): In this work, a highly sensitive electrochemical immunosensor for the determination of neutrophil gelatinase-associated lipocalin (NGAL) is reported. A miniaturized dual working electrode (WE) screen-printed electrochemical cell was employed as the transducer. The biosensing approach consisted of a sandwich format in which the specific immune-recognition event was detected using alkaline phosphatase as enzymatic label and a 3indoxyl phosphate/silver mixture as substrate. The analytical signal was based on the voltammetric stripping of the enzymatically deposited silver. The second WE functioned as a negative control. The described methodology achieved a low limit of detection (0.096 ng mL(-1); 0.192 fmol in a 50 mu L sample) in a 125-min assay (< 10 min hands-on time). The applicability of the proposed immunosensor was successfully verified through the analysis of spiked urine samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8