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Laboratory of Microbiology

Code: BIOL4061     Acronym: BIOL4061     Level: 400

Classification Keyword

Instance: 2022/2023 - 1S (of 06-10-2022 to 14-01-2023)

Active? Yes
Responsible unit: Department of Biology
Course/CS Responsible: Master's degree in Applications in Biotechnology and Synthetic Biology

Cycles of Study/Courses

Acronym No. of Students Study Plan Curricular Years Credits UCN Credits ECTS Contact hours Total Time
M:ABBS 1 The study plan from 2019 1 - 6 42 162

Teaching Staff - Responsibilities

Teacher Responsibility
Olga Maria Oliveira da Silva Lage

Teaching - Hours

Theoretical classes: 0,14
Laboratory Practice: 2,86
Type Teacher Classes Hour
Theoretical classes Totals 1 0,14
Olga Maria Oliveira da Silva Lage 0,14
Laboratory Practice Totals 1 2,86
Olga Maria Oliveira da Silva Lage 2,86

Teaching language

Suitable for English-speaking students


Bibliography search and organization of a database.

Discussion of scientific papers.

Improve knowledge on microbiological techniques used commonly in nowadays research.

Develop capacities to conceive structure and put in place an experimental work/design.

Organization of results obtained in the experimental work, elaboration of a poster as for presentation in a scientific meeting.

Elaboration of a manuscript.

Learning outcomes and competences

Development of capacities in cultural and molecular techniques in Microbiology

Working method



This course is mainly based on the planning and execution of a laboratorial study, treatment and analysis of the results and learning how to communicate them to the scientific community. This will be achieved by the preparation of a poster as for a scientific meeting and the elaboration of a manuscript. Culturing and genomic and/or proteomic molecular techniques will be performed in the laboratorial work. Several techniques frequently used in Microbiological studies namely Electron Microscopy, FISH, Brdu-FISH, CARD-FISH, MAR-CARD-FISH, Flow Cytometry, Fingerprinting Methods – DGGE, Pyrosequencing, q-PCR; real time-PCR and Microarrays will be studied focusing on their principles and applicability.

Mandatory literature

Reddy C. A., Beveridge T. J., Breznak J.A., Marzluf G., Schmidt T. M. & Snyder L.R; Methods for General and Molecular Microbiology, ASM Press, , 2007

Teaching methods and learning activities

Continuous evaluation during the course.

Poster for a communication in a conference (P)

Preparation of a scientific paper based on the laboratorial work developed (SP)

Oral presentation of a Microbiology topic (A))

NF = 30% (P) + 40% (SP) + 30% (A)

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation Weight (%)
Apresentação/discussão de um trabalho científico 30,00
Trabalho escrito 70,00
Total: 100,00

Amount of time allocated to each course unit

designation Time (hours)
Estudo autónomo 120,00
Frequência das aulas 42,00
Total: 162,00

Eligibility for exams


Calculation formula of final grade

Poster for a communication in a conference (P)

Preparation of a scientific paper based on the laboratorial work developed (SP)

Oral presentation of a Microbiology topic (A))

NF = 30% (P) + 40% (SP) + 30% (A)


Responsable for the discipline: Olga Maria Lage
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