Laboratory of Analytical Methods

Code:

Q4056

Acronym:

Q4056

Keywords
Classification	Keyword
OFICIAL	Chemistry

Instance: 2021/2022 - 2S

Active?	Yes
Responsible unit:	Department of Chemistry and Biochemistry
Course/CS Responsible:	Master in Advanced Methods and Accreditation in Chemical Analysis

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:MA3Q	8	Study plan from 2018/19.	1	-	6	42	162

Teaching language

Portuguese

Objectives

The course aims to provide thorough hands-on experience for students to perform and understand modern analytical instrumentation and execute validation procedures for chemical measurements.

 

Learning outcomes and competences

Upon successful completion of this course, students should be:

1 Able to apply the modern sample treatment and preparation techniques;

2 Able to apply the analytical procedures based on atomic and uv-vis spectroscopy, gas chromatography and high-performance liquid chromatography;

3 Able to perform chemical data analysis;

4 Able to perform a method validation;

5 Able to report a validation of an analytical method.

Working method

Presencial

Pre-requirements (prior knowledge) and co-requirements (common knowledge)

Basic knowledge of analytical chemistry, instrumental methods of analysis and validation methods

 

Program

1 Flame atomic absorption spectroscopy

1.1 Optimization of experimental parameters

1.2 Determination of metal content and study of interference

1.3 Method validation

2 UV-Vis spectroscopy

2.1 Optimization of experimental parameters

2.2 Analysis of inorganic anions in water

2.3 Method validation

3 Gas chromatography

3.1 Optimization of experimental parameters

3.2 Analysis of alcoholic drink using SPME and headspace sampling

3.3 Method validation

4 High-performance liquid chromatography

4.1 Optimization of experimental parameters

4.2 Analysis of compounds in chocolate

4.3 Method validation

 

Mandatory literature

Skoog Douglas A.; Principles of instrumental analysis. ISBN: 0-03-002078-6
Harris Daniel C.; Quantitative chemical analysis. ISBN: 0-7167-2508-8
Riley Cristopher M. 340; Development and validation of analytical methods. ISBN: 0-08042792-8

Complementary Bibliography

Ludwig Huber; Validation and Qualification in Analytical Laboratories, CRC Press LLC, 1999. ISBN: 1-57491-080-9
D. Brynn Hibbert; Quality Assurance for the Analytical Chemistry Laboratory, Oxford University Press, 2007. ISBN: 978-0-19-516212-7
Paul De Bièvre and Helmut Günzler (Eds); Validation in Chemical Measurement, Springer, 2010. ISBN: 978-3-642-05867-7

Teaching methods and learning activities

The teaching of this unit takes place through laboratory sessions.

In the laboratory sessions, the students carry out experimental works illustrative of the topics of the program, whose results and main conclusions will be presented and discussed orally.

Students will also have to search the literature for additional articles and book chapters relevant to experimental works carry out in the laboratory.

 

Software

Microsoft excel

keywords

Physical sciences > Chemistry > Instrumental analysis

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	35,00
Trabalho laboratorial	65,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	63,00
Frequência das aulas	42,00
Total:	105,00

Eligibility for exams

Terms of frequency: Student attendance to the practical classes and laboratory sessions is mandatory.
1 Those students whose attendance is lower than 3/4 of the laboratory sessions planned are considered without attendance.

2 All students must carry out at least two thirds of the experimental work of the laboratory sessions.

Calculation formula of final grade

Final mark= distributed evaluation (65%) + final exam (35%).

The distributed evaluation is based on laboratory reports and laboratory performance. Reports are designed to measure how well the students have learned the theory and application in instrumental methods validation.

The examination is designed to test students' competency in acquiring the intended learning outcomes of the laboratory course. Student abilities in the data treatment and interpretation will be evaluated.

For the students with final mark greater than 17, there will be an extra oral exam in order to confirm, or not, the final mark.

 

Examinations or Special Assignments

Are not provided

 

Internship work/project

not applicable

Special assessment (TE, DA, ...)

The special assessment shall be a laboratory examination

 

Classification improvement

Distributed evaluation can not be improved.

The final mark can be improved only by final exam