Evaluation of 3-hydroxy-benzo[a]pyrene levels in Nile tilapia (Oreochromis niloticus) after waterborne exposure to Benzo[a]pyrene

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Rey Salgueiro, L (Autor) Outra; Ferreira, M (Autor) Outra; reis-henriques, ma (Autor) FCUP

Revista

Título: Toxicological and Environmental Chemistry

Vol. 93

Páginas: 2040-2054

ISSN: 0277-2248

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-002-ZMV

DOI: 10.1080/02772248.2011.606108

Abstract (EN): 3-Hydroxy-benzo[a]pyrene (3-OH-BaP), a toxic compound with the ability to covalently bind with the macromolecules (proteins and DNA), is one of the major phenolic metabolites of benzo[a] pyrene (BaP). The purpose of this study was to evaluate the presence of 3-OH-BaP in the bile and plasma of Nile tilapia by HPLC with fluorescence detection, after waterborne exposure to BaP (10 and 100 mu g L-1). Metabolites were detected in bile and plasma, and conjugates of 3-OH-BaP (glucuronide and/or sulphate conjugates) were the majority forms in both biological fluids, glucuronide 3-OH-BaP being the main metabolite in bile. Our data suggest that extrahepatic tissues as intestine or gill are important in BaP metabolism and need to be the considered sources of metabolites released into the blood. Although, low levels of 3-OH-BaP in toxic form (free form) were detected in plasma, one cannot exclude the possibility of circulating the levels leading to adverse effects.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15