A critical comparison of analytical flow systems exploiting streamlined and pulsed flows

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Ana C B Dias (Autor) Outra; Joao L M Santos (Autor) FFUP; Jose L F C Lima (Autor) FFUP; Cristina M Quintella (Autor) Outra; Angelo M V Lima (Autor) Outra; Elias A G Zagatto (Autor) Outra

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 388 Nº 5

Páginas: 1303-1310

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 18 Citações

ISI Web of Science

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-004-8XM

DOI: 10.1007/s00216-007-1304-1

Resumo (PT): Multipumping (MPFS) and multicommuted (MCFS) flow systems relying on pulsed and laminar flows were critically compared. The mixing conditions and dispersion associated with both systems were evaluated by simulating the sample with bromocresol green. The molybdenum blue method for phosphate determination in soil extracts was also implemented in both flow systems. Furthermore, laser-induced fluorescence (LIF) was applied to visualize the dispersing sample; rhodamine B was used as the fluorescent species. The pulsed flow enhanced the mixing of the solutions involved, thus reducing reagent consumption (48 and 96 μl for MPFS and MCFS), and improving sampling rate (67 and 144 h−1 for MCFS and MPFS). For phosphate determination, results obtained with both systems were precise (r.s.d. < 0.5%; n  = 10) and accurate. Analyses of the absorbance vs time/space LIF plots revealed that exploitation of pulsed flow led to a pronounced radial dispersion and to a limited axial dispersion, typical aspects of turbulent flows. <br> <br> Keywords Flow analysis - Pulsed flow - Multipumping - Multicommutation <br> <a target="_blank" href="http://www.springerlink.com/content/r60502176627572j/?p=68ba5b16ce82425e9ba5a00e2dfea6ec&pi=11"> Texto integral </a> <br> <br>

Abstract (EN): Multipumping (MPFS) and multicommuted (MCFS) flow systems relying on pulsed and laminar flows were critically compared. The mixing conditions and dispersion associated with both systems were evaluated by simulating the sample with bromocresol green. The molybdenum blue method for phosphate determination in soil extracts was also implemented in both flow systems. Furthermore, laser-induced fluorescence (LIF) was applied to visualize the dispersing sample; rhodamine B was used as the fluorescent species. The pulsed flow enhanced the mixing of the solutions involved, thus reducing reagent consumption (48 and 96 mu l for MPFS and MCFS), and improving sampling rate (67 and 144 h(-1) for MCFS and MPFS). For phosphate determination, results obtained with both systems were precise (r.s.d.< 0.5%; n=10) and accurate. Analyses of the absorbance vs time/space LIF plots revealed that exploitation of pulsed flow led to a pronounced radial dispersion and to a limited axial dispersion, typical aspects of turbulent flows.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8