Factorial optimization of upstream process for Cyanobium sp. pigments production

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Pagels, F (Autor) Outra; Salvaterra, D (Autor) Outra; Amaro, HM (Autor) Outra; Lopes, G (Autor) Outra; Isabel Sousa Pinto (Autor) FCUP; Vasconcelos, V (Autor) Outra; Guedes, AC (Autor) Outra

Revista

Título: Journal of Applied Phycology

Vol. 32

Páginas: 3861-3872

ISSN: 0921-8971

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00S-Z99

DOI: 10.1007/s10811-020-02260-8

Abstract (EN): Cyanobacteria-based pigments, such as carotenoids and phycobiliproteins, have emerged in the last few years as products with great economical interest. However, only the production of a few strains has been optimized for large-scale productions. As photosynthetic components, pigments have their synthesis modulated by abiotic factors, such as pH, temperature and salinity, which can lead to a huge impact on cyanobacteria production. This work aimed the optimization of biomass and pigments production by Cyanobium sp. LEGE 06113, using a factorial Box-Behnken design for three abiotic factors-temperature (20-30 degrees C), pH (6.0-9.0) and salinity (NaCl, 10-30 g L-1). Biomass, photosynthetic activity, carotenoid and phycobiliprotein productivity and antioxidant capacity of acetonic and aqueous extracts were measured over time and plotted into quadratic models. Results revealed that temperature and pH had a more significant impact than salinity on Cyanobium sp. metabolism and it was possible to determine a significant quadratic model for all evaluated parameters. According to the factorial modelling, the optimal condition for biomass, carotenoids and phycobiliprotein productivity was obtained at 20 degrees C, pH 9.0 and 10 g L-1 of NaCl, as subsequently confirmed in experimental trials, with an observed productivity of 127.12 +/- 1.30 mg(DW) L-culture(-1) day(-1) for biomass; 2.04 +/- 0.51 mg(carot) L-culture(-1) day(-1) for total carotenoids; and 4.14 +/- 0.71 mg(phyco) L-culture(-1) day(-1) for total phycobiliproteins.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12