The 17 beta-hydroxysteroid dehydrogenase 4: Gender-specific and seasonal gene expression in the liver of brown trout (Salmo trutta f. fario)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

castro, lfc (Autor) Outra; rocha, mj (Autor) ICBAS; lobo-da-cunha, a (Autor) ICBAS; batista-pinto, c (Autor) Outra; machado, a (Autor) Outra; rocha, e (Autor) ICBAS

Revista

Título: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

Vol. 153

Páginas: 157-164

ISSN: 0305-0491

Editora: Elsevier

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 10 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-JDS

DOI: 10.1016/j.cbpb.2009.02.015

Abstract (EN): Previously, it was documented that liver peroxisomes display seasonal size changes in the adult Salmo trutta fario, especially in females (and negatively correlated with ovary maturation). It was then hypothesized that decreases in peroxisome size could be paralleled by changes in peroxisomal beta-oxidation and estradiol catabolism actions. The 17 beta-hydroxysteroid dehydrogenase 4 has been portrayed as playing an important role in both processes. To elucidate its function in the described peroxisomal pattern, we isolated the cDNA and predicted the protein sequence of the enzyme in that species. The seasonal gene expression pattern in both genders was addressed through quantitative PCR. Fish sampling was in post-spawning period, early and advanced gonad maturation, and pre-spawning. Males did not vary seasonally. As to females, a seasonal pattern was evidenced according to our previous hypothesis. We suggest that the decreased levels observed during vitellogenesis are related to lipid needs for ovary maturation, and, additionally, with the need of modulating estradiol titers.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8